Coding
hok

Part:BBa_K5291010:Design

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-10)
Revision as of 13:17, 10 August 2024 by Kortybones (Talk | contribs) (→‎Source)


hok/mok


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our design we choose a strong promoter that can be activated by citrate. The hok/sok system is a efficient system controlled by two promoters with different strength, which fits right in with our design with a strong promoter and a weak promoter.


Source

The plasmid R1 from Escherichia coli

References

[1]Gerdes, K., et al., Mechanism of postsegregational killing by the hok gene product of the parB system of plasmid R1 and its homology with the relF gene product of the E. coli relB operon. The EMBO Journal, 1986. 5(8): p. 2023-2029. [2]Gerdes, K., et al., The hok killer gene family in gram-negative bacteria. The New biologist, 1990. 2: p. 946-56. [3]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.