Coding

Part:BBa_K5237004

Designed by: Marik Mueller   Group: iGEM24_Heidelberg   (2024-08-10)
Revision as of 12:03, 10 August 2024 by Registry (Talk | contribs)

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fgRNA Entry vector MbCas12a-SpCas9

Basic leucine zipper from the yeast transcriptional activator GCN4. It recognises and binds to DNA as a dimer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 571
    Illegal SapI site found at 662
    Illegal SapI.rc site found at 280


[edit]
Categories
Parameters
None