Coding
Part:BBa_K5237004
Designed by: Marik Mueller Group: iGEM24_Heidelberg (2024-08-10)
fgRNA Entry vector MbCas12a-SpCas9
Basic leucine zipper from the yeast transcriptional activator GCN4. It recognises and binds to DNA as a dimer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 339
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 571
Illegal SapI site found at 662
Illegal SapI.rc site found at 280
[edit]
Categories
Parameters
None |