Regulatory
P_EIF5A
Part:BBa_K5088013
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
Translation initiation factor 5A - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1678
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1678
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 193
Illegal BglII site found at 889 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1678
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1678
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1930
[edit]
Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |