Regulatory
P_CLTC
Part:BBa_K5088007
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
Clathrin - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 245
Illegal EcoRI site found at 1549
Illegal XbaI site found at 1605
Illegal XbaI site found at 1631 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 245
Illegal EcoRI site found at 1549 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 245
Illegal EcoRI site found at 1549
Illegal BglII site found at 920
Illegal BglII site found at 1608 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 245
Illegal EcoRI site found at 1549
Illegal XbaI site found at 1605
Illegal XbaI site found at 1631 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 245
Illegal EcoRI site found at 1549
Illegal XbaI site found at 1605
Illegal XbaI site found at 1631 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |