Regulatory
P_FKBP4_5
Part:BBa_K5088006
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
FK506-binding protein 4/5 - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1400
Illegal XbaI site found at 59 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1400
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1400
Illegal BglII site found at 1664
Illegal XhoI site found at 1668 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1400
Illegal XbaI site found at 59 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1400
Illegal XbaI site found at 59 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |