Regulatory
P_MGRN1
Part:BBa_K5088003
Designed by: Dascha Khalfine Group: iGEM24_Marburg (2024-07-30)
E3 ubiquitin-protein ligase - Promoter+5'UTR from T. kok-saghyz
Contents
Background
Motivation
Use Cases
Lack of Endogenous Regulatory Parts
Political Context
Plant Synthetic Biology
Gene Structure in Plants
Cis-Regulatory Elements
Design and Characterization
Identification & Design Strategy
Genetic Context of the Part
Measurement
Dual Fluorescence Reporter Assay
Choice of Reporter Genes
Transient Transformation
Leaf Infiltration
Protoplast
Measurement Setup
Results
The Dandelion Toolbox
Overview
Dandelion Handbook
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 148
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 231
Illegal NheI site found at 242 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 148
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 148
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/plants/other
origin | Taraxacum kok-saghyz |