Coding
RCA
Part:BBa_K5291006:Design
Designed by: Rui Chen Group: iGEM24_BNUZH-China (2024-08-08)
acaP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 223
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 223
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 152
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 223
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.
Source
Rhodopseudomonas palustris CGA009
References
[1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966. [2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.