Regulatory
P_PTI1

Part:BBa_K5088000

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-30)
Revision as of 14:14, 7 August 2024 by Daschka (Talk | contribs)


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration
Protoplast

Measurement Setup


Results

The Dandelion Toolbox

Overview

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/plants/other
Parameters
originTaraxacum kok-saghyz