Coding

Part:BBa_K5226012:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-29)
Revision as of 01:39, 2 August 2024 by Admin (Talk | contribs) (→‎Design Notes)

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Vib-gcvT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component(BBa_K) formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 618bp and 843bp of Vib-gcvT from g/c to a/t.

Source

Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1371492&to=1372610）

References