Coding

Part:BBa_K5226008:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-28)
Revision as of 13:52, 1 August 2024 by Admin (Talk | contribs) (→‎Design Notes)


TD-sdaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 646
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of illegal sites in this component that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 1249bp base pair of TDsdaA from g/c to t/a.

Source

Sequence source is from Halomonas sp. TD01 strain, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=2231433&to=2232800）

References