Coding

Part:BBa_K5226004:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-27)
Revision as of 13:49, 1 August 2024 by Admin (Talk | contribs) (→‎Design Notes)


TD-gcvT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 627
    Illegal PstI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of illegal sites in this component that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the base pairs at 600bp and 1023bp of TD gcvT from g/c to t/a.

Source

Sequence source is from Halomonas sp. TD01 strain, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=3301149&to=3302261）

References