Part:BBa_K5226003

TD-lpd

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

TD-Lpd is dihydrolipoyl dehydrogenase.The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the conversion of dihydrolipoyllysine into lipoyllysine during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1306
  Illegal XbaI site found at 579
  Illegal PstI site found at 449
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1306
  Illegal PstI site found at 449
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1306
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1306
  Illegal XbaI site found at 579
  Illegal PstI site found at 449
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1306
  Illegal XbaI site found at 579
  Illegal PstI site found at 449
  Illegal AgeI site found at 361
  Illegal AgeI site found at 1144
  
• 1000
  COMPATIBLE WITH RFC[1000]