Coding

Part:BBa_K5226003:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-27)
Revision as of 13:38, 1 August 2024 by Admin (Talk | contribs) (→‎Design Notes)


TD-lpd


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of illegal sites in this component that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the 374bp base pair of TD lpd from g/c to t/a.

Source

Sequence source is from Halomonas sp. TD01 strain, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=96413&to=97852）

References