Coding
P.Stu

Part:BBa_K5078000:Design

Designed by: Eric Domyan   Group: iGEM24_UVU-Utah-2   (2024-07-31)
Revision as of 17:58, 31 July 2024 by Edomyan (Talk | contribs) (→‎References)

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Nos Z gene from P. stutzeri


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 541
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1303
    Illegal PstI site found at 541
    Illegal NotI site found at 145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1520
    Illegal BamHI site found at 467
    Illegal XhoI site found at 34
    Illegal XhoI site found at 1588
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 541
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 541
    Illegal NgoMIV site found at 1075
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtained from Chlamycollection.org


Source

Chlamy MoClo kit

References

Crozet et al. (2018) Birth of a phototsynthetic chassis: A MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii. ACS Synth Biol. 7: 2074-2086