Part:BBa_K5226014
Vib-gcvP
Introduction
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
Usage and Biology
Vib-GcvP is glycine dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine during the glycine cleavage system(C2M).This part is used in BBa_xx and BBa_xx.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1629
Illegal NheI site found at 2226
Illegal PstI site found at 2237 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 353
Illegal BglII site found at 1638 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 1000COMPATIBLE WITH RFC[1000]
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