Coding
Part:BBa_K5226014:Design
Designed by: Yujiao Yang Group: iGEM24_SCUT-China-A (2024-07-29)
Vib-gcvP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1629
Illegal NheI site found at 2226
Illegal PstI site found at 2237 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 353
Illegal BglII site found at 1638 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 546
Illegal XbaI site found at 1602
Illegal PstI site found at 2237 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI (https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1375714&to=1378578)