Part:BBa_K5226009
Vib-ftl
Introduction
One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).
Usage and Biology
Vib-Ftl is formate-tetrahydrofolate ligase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein catalyzes the formation of 10-formyltetrahydrofolate from formate and tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) . This part is used in BBa_xx and BBa_xx
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1059
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1059
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1059
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1059
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1059
Illegal NgoMIV site found at 325 - 1000COMPATIBLE WITH RFC[1000]
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