Coding
MaSp1

Part:BBa_K5143001:Design

Designed by: Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-07-28)
Revision as of 12:21, 28 July 2024 by Jesslyon (Talk | contribs) (→‎References)

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MaSp1 : a spider silk protein used as a bioglue, optimised for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for DNA synthesis and for Saccharomyces cerevisiae.



Source

The source of the part will be its sequence which was retrieved from Yan et al., 2023.

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.