Coding

Part:BBa_K5226003

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-27)
Revision as of 13:36, 27 July 2024 by Registry (Talk | contribs)

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TD-lpd

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1306
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1306
    Illegal XbaI site found at 579
    Illegal PstI site found at 449
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]


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