Primer

Part:BBa_K4615006:Design

Designed by: Xinyi (Marry) Xuan   Group: iGEM23_Toronto   (2023-10-12)
Revision as of 15:59, 12 October 2023 by 20mxuan (Talk | contribs)

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Reverse primer to insert 20 bp of sgRNA of frdA into pTargetF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained this part by introducing 20 bp of sgRNA within the tpiA genome that guides the Cas9 protein to cut and create strand breaks.

Annealing Temperature: 48C

GC:35%

Source

The 20bp of sgRNA was generated using CHOPCHOP and analyzed based on various factors such as GC content, self-complementarity and efficiency.

References