Primer

Part:BBa_K4615001:Design

Designed by: Xinyi (Marry) Xuan   Group: iGEM23_Toronto   (2023-10-12)
Revision as of 15:57, 12 October 2023 by 20mxuan (Talk | contribs)

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Reverse primer to insert 20 bp of sgRNA of tpiA into pTargetF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained this two parts by introducing 20 bp of sgRNA within the tpiA genome that guides the Cas9 protein to cut and create strand breaks.

Forward Melting temp: 45.7C

GC 50%


Source

The 20bp of sgRNA was generated using CHOPCHOP and analyzed based on various factors such as GC content, self-complementarity and efficiency.

References