Regulatory

Part:BBa_K4725001

Designed by: Fung Ching Nam   Group: iGEM23_HKUST   (2023-10-12)
Revision as of 15:57, 12 October 2023 by Lrsalim (Talk | contribs)


-- No description --

PsinI is an endogenous promoter in B. subtilis. It is activated by phosphorylated spo0A, a master regulator in B. subtilis. Its expression can be induced by potassium efflux using potassium ionophores such as valinomycin and cereulide. In our case, 0.3 µM of valinomycin is enough to trigger this promoter when B. subtilis is grown in BHI medium with pH 8.5.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None