Regulatory
pLux/Tet

Part:BBa_K176000

Designed by: Danqian Liu, Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-05-06)
Revision as of 02:36, 21 October 2009 by Dqliu (Talk | contribs)

pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

sense two inputs, activated by LuxR/3OC6HSL and repressed by tetR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

MEASUREMENT

K176000, the hybrid promoter，is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.

AHL&time-->K176000

K176026[1]: pCon 0.70->luxR+pLux/Tet->GFP

K176126[2]: pCon 0.36->luxR+pLux/Tet->GFP

K176128[3]: pCon 0.15->luxR+pLux/Tet->GFP

K176130[4]: pCon 0.04->luxR+pLux/Tet->GFP

AHL&aTc-->K176000

K176071[5]: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176075[6]: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176078[7]: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFP



[edit]
Categories
Parameters
n/apLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS