Generator

Part:BBa_K218011

Designed by: Sung Yong (Kevin) Shin   Group: iGEM09_Calgary   (2009-10-16)
Revision as of 01:35, 21 October 2009 by Ssy080 (Talk | contribs)

LuxO inducible GFP

LuxO when phosphorylated binds to Pqrr4, GFP is expressed. Alternatively, LuxO D47E mutant can also bind to the Pqrr4 and induce GFP production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 945


Pictures

Characterization of the reporter (Pqrr4 + I13500) circuit

The functionality of the reporter was tested by measuring the fluorscence of reporter together with LuxO D47E (K218017) mutant, and this fluorscence was compared to the fluorscence of our positive control (R0040 + I13500). The following is the protocol of the fluorescence reading.
1. Power on the Bio-tec Synergy HT plate reader, or another plate reader, and KC4 application.
2. On a black 96 well plate, aliquot samples in required wells.
3. Measure OD600 by using the following settings:
Reader: absorbance
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Categories
Parameters
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