Coding

Part:BBa_K4805009:Design

Designed by: Yuhan Gan   Group: iGEM23_LINKS-China   (2023-10-12)
Revision as of 15:22, 12 October 2023 by Registry (Talk | contribs)

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pTDH3-BmeTC-FL-BmeTC_Y167A,D373C-tTDH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1357
    Illegal NheI site found at 3262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 760
    Illegal BamHI site found at 2665
    Illegal XhoI site found at 4472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Its DNA sequences have been optimized which can be expressed in S. cerevisiae.


Source

DNA synthesis

References