Coding

Part:BBa_K4805002:Design

Designed by: Tianyin Zhao   Group: iGEM23_LINKS-China   (2023-10-12)
Revision as of 15:10, 12 October 2023 by WilliamZhao (Talk | contribs)

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BmeTC-FL-BmeTC Y167A,D373C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 676
    Illegal NheI site found at 2581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 79
    Illegal BamHI site found at 1984
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

used to produce higher yield of ambrein with squalene as substrate


Source

Bacillus megaterium

References