Part:BBa_K4620016
Skr-BURP tryptic peptide with Y- deletion containing the core peptide
ADNVLFPSYAAR is a modification of ADNVLFYPSYAAR, a peptide formed after tryptic digestion of Skr-BURP protein. It contains core peptide fragment VLFYPSY. VLFYPSY is a peptide that can be cyclised by BURP domain protein from African clubmoss (Selaginella kraussiana) (BBa_K460000), forming selanine A. This side chain macrocyclisation reaction is catalysed by copper (II) ions. Cyclisation happens between the OH-group of tyrosine and Cβ of leucine or Cβ of tyrosine. A bicyclic compound is formed. As of this day, mechanism of this reaction has not been fully characterised. In the nature, ADNVLFYPSYAAR is conjugated to the N-terminal domain of Skr-BURP. iGEM 2023 team Latvia-Riga produced this peptide separately in order to make trans-cyclisation reaction where the cyclisation substrate is not attached to the catalytic domain. We chose substrate a little bit longer than the core domain in order to make expression and purification easier.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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