Part:BBa_K4613888

pSB1C3-tra-lysis

This composite part confers the ability for engineered bacteria to undergo self-destruction when perceiving the high cell density. When we add 1 mM IPTG, TraI protein will be expressed and take charge of the formation of ligand, then TraR, will bind to the ligand and become active and combine to P_tra, increasing the expression of downstream lysis E.

Fig. 1 Schematic illustration of the strategy for autolysis circuit.

Fig. 2 (a) The plasmid map of pSB1C3-tra-lysis. (b) The results of triple autolysis groups：pSB1C3-tra-lysis (with IPTG), pSB1C3-tra-lysis (without IPTG), and empty pSB1C3.

Semi-interpenetrating polymer networks (sIPN)'s function is to encapsulate engineered bacteria.

Fig. 5 Living fabrication of semi-IPN.

If you desire to know our future plan to improve this composite part, navigate NAU-CHINA 2023 Projects Results.

Reference

1. White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
2. White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.
3. Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
4. Henrich B, Lubitz W, Plapp R.Lysis of Escherichia coli by induction of cloned phi X174 genes[J].Mol Gen Genet,1982, 185 (3): 493-7.

Young K D, Young R.Lytic action of cloned phi X174 gene E[J].J Virol,1982, 44 (3): 993-1002.

1. Bernhardt T G, Roof W D, Young R.Genetic evidence that the bacteriophage phi X174 lysis protein inhibits cell wall synthesis[J].Proc Natl Acad Sci U S A,2000, 97 (8): 4297-302.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 429
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 429
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 429
  Illegal BglII site found at 14
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 429
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 429
  Illegal NgoMIV site found at 1592
  
• 1000
  COMPATIBLE WITH RFC[1000]