Coding

Part:BBa_K4645008:Design

Designed by: Meng Sun   Group: iGEM23_HZAU-China   (2023-10-10)
Revision as of 14:09, 12 October 2023 by Ruixuan Zhu (Talk | contribs) (→‎Source)

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A chimeric lysozyme with secretory ability.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 816
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 184
    Illegal AgeI site found at 268
    Illegal AgeI site found at 583
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

OmpA is added to the N-terminus of the ClyR protein.

Source

The catalytic domain come from Streptococcus virus CI and the cell-wall binding domain come from Streptococcus suis.

References

[1] Xu J, Yang H, Bi Y, Li W, Wei H, Li Y. Activity of the Chimeric Lysin ClyR against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models. Viruses. 2018 Jul 20;10(7):380. [2] Yang H, Linden SB, Wang J, Yu J, Nelson DC, Wei H. A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method. Sci Rep. 2015 Nov 26;5:17257.