Part:BBa_K4844007
Low phosphate phytosensor with low noise amplifier gene circuit
Usage: Plants usually have severe responses under the pressure of low phosphate. Some genes will be turned on to allow plants to better adapt to this situation. Therefore, in order to build a low-cost, real-time soil phosphate sensor, we first found a low phosphate response promoter in Maize which was reported by Jianrong Bai in 2018. Based on Bai's result, a 1502bp length promoter P1502-ZmPHR1 (BBa_K4844002, a new part submitted by our team in 2023) shows the strongest promoting strength under low phosphate pressure. Therefore, we decided to choose it as our low phosphate detection part. The sensor vector will be transformed into plants later with our "TTTT" system. (More about TTTT can be found at https://2023.igem.wiki/sz-shd/plant )
When the plant was under low phosphate stress. The P1502-ZmPHR1 promoter will up-regulate its' expression, the signal then enters the amplifier system, which eventually yields a large quantity of super bright eyGFP_UV (BBa_K4844000, new basic part submitted by our team in 2023) protein that can be seen under UV light with the naked eye.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 709
Illegal NheI site found at 2259
Illegal NheI site found at 5016
Illegal NheI site found at 5358
Illegal NheI site found at 8395 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 7057
Illegal BglII site found at 10413
Illegal BamHI site found at 6823 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5012
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4995
Illegal SapI site found at 11505
Illegal SapI.rc site found at 5132
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