Coding

Part:BBa_K4805012

Designed by: Yuhan Gan   Group: iGEM23_LINKS-China   (2023-10-12)
Revision as of 14:05, 12 October 2023 by Registry (Talk | contribs)

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pPGK1-SaCPR2-tPGK1-pTDH3-CYP736A167-tTDH1

SaCPR2 is a cytochrome P450 reductase from S. album (GenBank Accession: KC842188) . CYP736A167 is a cytochrome P450 enzyme (GenBank Accession: KU169302.1) specifically produces the Z type santalols. And the oxidation of santalene to Z-santalol by CYP736A167 relies on the cooperation of SaCPR2(a cytochrome P450 reductase from S. album).

This part is composite of SaCPR2 and CYP736A167 under the control of pTDH3 and pPGK1. It can be used to inspire teams to build the pathway of catalyzing santalene into santalol. It belongs to the terpenoid part collection we have established for the terpenoids production of santalol and ambrein in S. cerevisiae, which includes BBa_K4805000-BBa_K4805012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1932
    Illegal NheI site found at 2032
    Illegal NheI site found at 5001
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4174
    Illegal BglII site found at 4759
    Illegal XhoI site found at 2818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3072
    Illegal BsaI.rc site found at 4184
    Illegal SapI site found at 4303


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Parameters
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