Device

Part:BBa_K4990015

Designed by: Liang Chen   Group: iGEM23_CPU-CHINA   (2023-10-12)
Revision as of 13:53, 12 October 2023 by Registry (Talk | contribs)

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HokB time bomb

To ensure patient safety, a time bomb based on a weak RBS was designed to prevent excessive proliferation of engineering bacteria. Once the engineering bacteria has permeated the tumor microenvironment, the expression of toxin is automatically activated. The accumulation of toxin is slow due to the effect of the weak RBS, thereby preventing immediate death of the engineering bacteria. During this time frame, it is possible to engineer bacteria that can release DEH we design, and successfully accomplishing the mission of dual killing. As time passes, the accumulation of toxin will eventually reach a certain threshold, at which point the engineered bacteria will be killed under their own action, thereby completing the suicide process in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None