Coding

Part:BBa_K4614002:Design

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-09-30)
Revision as of 13:49, 12 October 2023 by FMD (Talk | contribs) (→‎Source)

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Lnak-R5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5.INP(encoded by Lnak) is an ice nucleoprotein encoded by Lnak from Pseudomonas syring, which we use to surface R5 with E. coli.

Source

IPN(encoded by Lnak) comes from the genome of Pseudomonas syringae.

References

[1]薛双红.(2017).基于细菌表面展示技术的功能性无机材料合成研究(硕士学位论文,武汉理工大学).https://kns.cnki.net/KCMS/detail/detail.aspx?dbname=CMFD201901&filename=1019808772.nh