Composite

Part:BBa_K4614003:Design

Designed by: Miaodi Feng   Group: iGEM23_CAU-China   (2023-10-12)
Revision as of 13:34, 12 October 2023 by FMD (Talk | contribs) (→‎Design Notes)

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T7-RBS-Lnak-R5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 572
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 572
    Illegal NgoMIV site found at 115
    Illegal NgoMIV site found at 448
    Illegal AgeI site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

R5 is a short 19-amino acid peptide with multiple Lys catalytic groups that plays an important role in the formation of the siliceous cell wall of diatoms, and we wanted to use it to hydrolyze TEOS to generate silica and deposit silica on the surface of R5.INP is an ice nucleoprotein encoded by Lnak from Pseudomonas syring, which we use to surface R5 with E. coli.

Source

not have

References

[1]薛双红. 基于细菌表面展示技术的功能性无机材料合成研究[D].武汉理工大学,2019.