Part:BBa_K4805003

ClSS

Description

ClSS is a santalene synthase from Clausena lansium (Genbank Accession: ADR71055.1 ), which can catalyze the conversion of FPP(farnesyl diphosphate) into santalene. Compared with other santalene synthase, ClSS is capable of producing the most a-santalene(Jiachen Z. et al, 2022). In our project we expressed this heterologous gene in the genome of S. cerevisiae in order to yield santalene.
Our part can inspire other teams to produce santalene from FPP. It belongs to the terpenoid part collection we have established for the production of santalol and ambrein in S. cerevisiae, which includes BBa_K4805000-BBa_K4805012.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1072
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Hua G; Hu Y; Yang C; Liu D; Mao Z; Zhang L; Zhang Y; (2018) Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay, Analytical biochemistry. Available at: https://pubmed.ncbi.nlm.nih.gov/29438678/ Zha, W., Zhang, F., Shao, J. et al. Rationally engineering santalene synthase to readjust the component ratio of sandalwood oil. Nat Commun 13, 2508 (2022). https://doi.org/10.1038/s41467-022-30294-8 Zhang, Jia, et al. “Sesquiterpene Synthase Engineering and Targeted Engineering of α-Santalene Overproduction in Escherichia Coli.” Journal of Agricultural and Food Chemistry, vol. 70, no. 17, 24 Apr. 2022, pp. 5377–5385, https://doi.org/10.1021/acs.jafc.2c00754.ed.ncbi.nlm.nih.gov/31940436/