DNA

Part:BBa_K4765130

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-02)
Revision as of 13:24, 12 October 2023 by YijunWang (Talk | contribs)


shuttle test contributed by Fudan iGEM 2023


Introduction

This composite part is designed to assess whether pRAP can function in cyanobacteria. It includes StayGold, stem-loop, Twister P1, and smURFP. We connect this composite part into a self-replicating shuttle vector that can replicate and function in both E. coli and cyanobacteria[1].. When both red and green fluorescence can be expressed, it can be concluded that pRAP is functional in cyanobacteria. This provides a convenient expression system for genetic modification in cyanobacteria.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1181
    Illegal BsaI.rc site found at 1201


  1. ↑ Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology, 162(12), 2029–2041. https://doi.org/10.1099/mic.0.000377
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