Composite

Part:BBa_K4601241:Design

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)
Revision as of 13:20, 12 October 2023 by Do-riane (Talk | contribs)

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AmpR expression cassette under the control of the J23110 promoter and of the Na+ RiboSwitch v1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

DNA synthesis, PCR amplifications and Golden Gate assembly

References