Composite

Part:BBa_K4584002

Designed by: Asmae Ait Abdallah   Group: iGEM23_KCL-UK   (2023-10-12)
Revision as of 12:36, 12 October 2023 by Asmae (Talk | contribs)


New composite part medium promoter, operator and strong RBS (+GFP)

New composite part Medium promoter BBa_J23110, LldR operator BBa_K4584001, strong RBS B0034 and GFP BBa_E0040

bba-k4584002-map.png

We designed and ordered a new composite BioBrick BBa_K4584002 with cloning linkers shown in yellow, medium promoter BioBrick BBa_J23110 (shown in magenta), LldR operator region BioBrick BBa_K4584001 (shown in grey), strong RBS BioBrick BBa_B0034 (shown in teal) and GFP BioBrick BBa_E0040 (shown in green), scar sequences underlined. sequence-composite-pt-1.png

Medium Promoter https://parts.igem.org/Part:BBa_J23110 LldR operator https://parts.igem.org/Part:BBa_K4584001 Strong RBS https://parts.igem.org/Part:BBa_B0034 GFP https://parts.igem.org/Part:BBa_E0040


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


[edit]
Categories
Parameters
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