Composite

Part:BBa_K4645020:Design

Designed by: Meng Sun   Group: iGEM23_HZAU-China   (2023-10-11)
Revision as of 12:36, 12 October 2023 by Felicitysm (Talk | contribs)


The two-component system of S. mutans senses the CSP to regulate the expression of ClyR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 627
    Illegal EcoRI site found at 1985
    Illegal XbaI site found at 3288
    Illegal PstI site found at 1027
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 627
    Illegal EcoRI site found at 1985
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1027
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 627
    Illegal EcoRI site found at 1985
    Illegal XhoI site found at 3240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 627
    Illegal EcoRI site found at 1985
    Illegal XbaI site found at 3288
    Illegal PstI site found at 1027
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 627
    Illegal EcoRI site found at 1985
    Illegal XbaI site found at 3288
    Illegal PstI site found at 1027
    Illegal AgeI site found at 2608
    Illegal AgeI site found at 2692
    Illegal AgeI site found at 3007
    Illegal AgeI site found at 3190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1823
    Illegal BsaI.rc site found at 2262


Design Notes

Gene synthesis and homologous recombination.


Source

comDE and related promoter PnlmC are derived from the Streptococcus mutans genome.

References

[1] Zhang K, Ou M, Wang W, Ling J. Effects of quorum sensing on cell viability in Streptococcus mutans biofilm formation. Biochem Biophys Res Commun. 2009 Feb 20;379(4):933-8. [2] Xu J, Yang H, Bi Y, Li W, Wei H, Li Y. Activity of the Chimeric Lysin ClyR against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models. Viruses. 2018 Jul 20;10(7):380. [3] Yang H, Linden SB, Wang J, Yu J, Nelson DC, Wei H. A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method. Sci Rep. 2015 Nov 26;5:17257.