Coding

Part:BBa_K4989000:Design

Designed by: Athanasia Arampatzi   Group: iGEM23_Thrace   (2023-10-02)
Revision as of 12:28, 12 October 2023 by Atharab (Talk | contribs) (→‎Source)

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Acetyl-CoA C-acetyltransferase (thiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 984
    Illegal BamHI site found at 849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal AgeI site found at 873
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.

Source

Our part was synthetically produced but the sequence comes from Coprococcus sp. L2-50 DSM.

References

[1]Petra Louis, Sheila I. McCrae, Cédric Charrier, Harry J. Flint, Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer, FEMS Microbiology Letters, Volume 269, Issue 2, April 2007, Pages 240–247,https://doi.org/10.1111/j.1574-6968.2006.00629.x [2]“Butyrate-Producing Bacterium L2-50 Putative Fe-S Oxidoreductase Gene, Partial Cds; Thiolase, Crotonase, Beta Hydroxybutyryl-CoA Dehydrogenase, Butyryl-CoA Dehydrogenase, Electron Transfer Flavoprotein Beta-Subunit, and Electron Transfer Flavoprotein Alpha-Subunit Genes, Complete Cds; and Putative Multidrug Efflux Pump Gene, Partial Cds.” NCBI Nucleotide, July 2016, https://www.ncbi.nlm.nih.gov/nuccore/DQ987697.1/