Coding

Part:BBa_K4825049

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:22, 12 October 2023 by Aisy (Talk | contribs)

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COX4-LEU4^S547∆

This compostie part contains the part COX4 BBa_K4825014 and LEU4^S547∆ BBa_K4825011. COX4 encodes cytochrome c oxidase subunit IV in S. cerevisiae, located in the mitochondria. LEU4^S547∆ encodes 2-isopropylmalate synthase, which is involved in leucine biosynthesis pathway to catalyze the conversion of 2-ketoisovalerate into alpha-isopropylmalate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 549
    Illegal SpeI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1756
    Illegal BamHI site found at 702
    Illegal BamHI site found at 1668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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