Coding

Part:BBa_K4451010

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)
Revision as of 12:03, 12 October 2023 by Koxley2 (Talk | contribs)


T4 AsiA

BBa_K4451010 encodes the AsiA protein from the T4 phage. This protein has been reported to interact with sigma-70 factor and inhibits recognition of sigma-70 promoters (Ouhammouch et al., 1995).

Description

A transcriptional inhibitor from T4 phage, which causes growth rate suppression in E. coli. The AsiA gene encodes an anti-σ factor, which is able to bind to σ70 and prevent the RNA polymerase σ70 holoenzyme from recognising σ70 specific promoters, thus enacting a transcriptional downregulation of housekeeping genes and subsequently causing growth rate suppression. AsiA further enables the transcription of phage middle genes as part of the T4 infection cycle (J. Liu et al., 2004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None