Part:BBa_K190015:Experience

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User Reviews

UNIQb71e8a6b2ff48a3e-partinfo-00000000-QINU

•• iGEM Groningen 2009

The part (BBa_K190015) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression.

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•• iGEM Groningen 2009

Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1). Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015)

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UNIQb71e8a6b2ff48a3e-partinfo-00000004-QINU