Plasmid

Part:BBa_K243037

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 19:44, 20 October 2009 by Timo (Talk | contribs)

pBAD
The cloning vector pBAD has the RFC25 restriction sites and an ampicilin resistance. It is inducible by arabinose and when the inserted part has an RBS, it can be used has an expression vector. The vector has a certain stability against toxic products.
PBADvetocorplasmapper.png
plasMap


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2434
    Illegal SpeI site found at 3052
    Illegal PstI site found at 3066
    Illegal NotI site found at 2440
    Illegal NotI site found at 3059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2434
    Illegal BamHI site found at 2338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3052
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2434
    Illegal suffix found in sequence at 3042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3106
    Illegal BsaI site found at 3284
    Illegal BsaI site found at 4352
    Illegal SapI site found at 2155
    Illegal SapI.rc site found at 783
    Illegal SapI.rc site found at 2949


[edit]
Categories
Parameters
None