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Coding
Part:BBa_K5002345
Designed by: Haoran Yang Group: iGEM23_Squirrel-Beijing-I (2023-10-12)
a cellulose synthesis operon+plant cellulose discomposing genes
The discharge of printing and dyeing wastewater has a very bad impact on the environment, we wanted to reduce the content of harmful substances in printing and dyeing wastewater to the standard of discharge. Therefore, we decided construct a cellulose synthesis operon and a group of plant cellulose discomposing genes. Cellulose synthase (acsAB) is composed of two domains: acsAB is the catalytic domain, and the cyclic di-GMP binding domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 185
Illegal BglII site found at 1764 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 799
Illegal NgoMIV site found at 1803
Illegal NgoMIV site found at 1872
Illegal NgoMIV site found at 1891
Illegal AgeI site found at 1750
Illegal AgeI site found at 2383
Illegal AgeI site found at 3382
Illegal AgeI site found at 4231 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1369
Illegal BsaI.rc site found at 2617
Illegal SapI.rc site found at 439
[edit]
Categories
Parameters
None |