Coding
Part:BBa_K4808008
Designed by: Zhao Guichun Group: iGEM23_AIS-China (2023-10-11)
thrABC
In Escherichia coli, thrA, thrB and thrC are arranged adjacently on the chromosome to form the thrABC operon. The thrABC operon controls several key enzymes, from aspartate to L-threonine synthesis. Research on thrABC expression and regulation is important for L-threonine synthesis and the downstream products, L-isoleucine and L-glycine. Overexpression of the thrABC operon in a bacterial strain increased L-threonine production significantly.
In our project, we increased the copy number of thrABC in E. coli to increase the production of a-KB.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3506
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3506
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3506
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3506
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3506
Illegal NgoMIV site found at 403
Illegal NgoMIV site found at 3357
Illegal AgeI site found at 912
Illegal AgeI site found at 1839 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3614
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Categories
Parameters
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