Coding

Part:BBa_K4808008

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-11)
Revision as of 11:25, 12 October 2023 by Yu Jun (Talk | contribs)


thrABC

In Escherichia coli, thrA, thrB and thrC are arranged adjacently on the chromosome to form the thrABC operon. The thrABC operon controls several key enzymes, from aspartate to L-threonine synthesis. Research on thrABC expression and regulation is important for L-threonine synthesis and the downstream products, L-isoleucine and L-glycine. Overexpression of the thrABC operon in a bacterial strain increased L-threonine production significantly.

In our project, we increased the copy number of thrABC in E. coli to increase the production of a-KB.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3506
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3506
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 3357
    Illegal AgeI site found at 912
    Illegal AgeI site found at 1839
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3614


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Categories
Parameters
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