Coding

Part:BBa_K4770026

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)
Revision as of 11:15, 12 October 2023 by Andreacami (Talk | contribs)

NR_Nitrate_Reductase_Level_M

Description and Biology

Level Ms are the assembly of different transcriptional units into the same vector. All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette. In this case, we are using pCM1:NR With this vector, we aim to see the effects of overexpressing NR constitutively and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.

How can this part be used

This Level M is aimed to be assembled with HygroR cassette in position 4, NR Level1 in position 5, mCherry cassette in position 6, and mVenus cassette in position 7. Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com

Source of this part

Position 4: Hygro R cassette BBa_K4770016 Position 5: pCM1 NR BBa_K4770018 Position 6: mCherry cassette BBa_K4770017 Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5726
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
    Illegal NotI site found at 9551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3882
    Illegal PstI site found at 5146
    Illegal PstI site found at 8289
    Illegal PstI site found at 9815
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 3771
    Illegal NgoMIV site found at 4365
    Illegal NgoMIV site found at 4980
    Illegal NgoMIV site found at 6097
    Illegal NgoMIV site found at 10292
    Illegal AgeI site found at 3543
    Illegal AgeI site found at 5856
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None