Composite

Part:BBa_K4613888

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 11:10, 12 October 2023 by Jiandong (Talk | contribs)


pSB1C3-tra-lysis

This composite part confers the ability for engineered bacteria to undergo self-destruction when perceiving the high cell density. When we add IPTG, TraI protein will be expressed and take charge of the formation of ligand, then TraR, will bind to the ligand and become active and combine to Ptra, increasing the expression of downstream lysis E.

Fig.1 Schematic illustration of the strategy for autolysis circuit. .

Fig.2 a. The plasmid map of pSB1C3-tra-lysis. b. The results of triple autolysis groups：pSB1C3-tra-lysis (with IPTG), pSB1C3-tra-lysis (without IPTG), and empty pSB1C3.

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Reference

  1. White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
  2. White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.
  3. Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
  4. Henrich B, Lubitz W, Plapp R.Lysis of Escherichia coli by induction of cloned phi X174 genes[J].Mol Gen Genet,1982, 185 (3): 493-7.

Young K D, Young R.Lytic action of cloned phi X174 gene E[J].J Virol,1982, 44 (3): 993-1002.

  1. Bernhardt T G, Roof W D, Young R.Genetic evidence that the bacteriophage phi X174 lysis protein inhibits cell wall synthesis[J].Proc Natl Acad Sci U S A,2000, 97 (8): 4297-302.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 429
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 429
    Illegal NgoMIV site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None