Coding

Part:BBa_K4694002

Designed by: Becky Davies   Group: iGEM23_Exeter   (2023-09-25)
Revision as of 10:58, 12 October 2023 by ChloeS (Talk | contribs)


His-QQ-7

Usage and Biology

The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as Candida albicans and Staphylococcus epidermidis (Weiland-Brauer et al., 2019). The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.

This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT.

Characterisation

References

Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of Candida albicans and Staphylococcus epidermidis. PLoS One, 14, e0211366.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None