Composite

Part:BBa_K243017

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 18:55, 20 October 2009 by Timo (Talk | contribs)

Strep-DigA-Split Linker-Fok_a

This part is the counter part to BBa_K243010,it inhabits the active cutting site of our universal endonuclease. We prefer this part because it has an other combiantion of basic parts than His-FluA-Split-Fok_i, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] The purification is made with a Streptavidin column and the linkage is done by dioxigenin binding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1132


[edit]
Categories
Parameters
n/aStrep-DigA-Split Linker-Fok_a